Effect of nerve growth factor on changes of myelin basic protein and functional repair of peripheral nerve following sciatic nerve injury in rats.
- Author:
Yang SHAO
1
;
Haihan MA
;
Yamin WU
;
Hengsheng CHEN
;
Lin ZENG
;
Min LI
;
Zaiyun LONG
;
Yingyu LI
;
Hengwen YANG
Author Information
- Publication Type:Clinical Trial
- MeSH: Animals; Evoked Potentials; Female; Immunohistochemistry; Myelin Basic Protein; metabolism; Nerve Growth Factor; pharmacology; Peripheral Nerve Injuries; Peripheral Nerves; metabolism; Rats; Rats, Wistar; Sciatic Nerve; injuries; metabolism
- From: Chinese Journal of Traumatology 2002;5(4):237-240
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the therapeutic effect of nerve growth factor (NGF) on changes of myelin basic protein (MBP) and functional repair of sensory and motor nerve following sciatic nerve injury.
METHODSThe sciatic nerves of rats were injured by sectioning with shaver,and divided into 3 groups: NGF group (Group A), group of normal saline solution (Group B), untreated group (Group C). The time point of observation was at the 4th week after operation. Sensory evoked potential (SEP) and motor evoked potential (MEP) were detected by Model WD-4000 nerve potential working diagnosis system. Immunohistochemical analysis was used for identification of MBP.
RESULTSThe latency of SEP in the Group A at the 4th week after operation was shorter than that in the Group B (P<0.05). The MEP was elicited in 76% of the Group A and was higher than that in the Group B. Results of immunohistochemistry showed that there were less MBP-positive cells in the Group A than in the Group B in one and four weeks respectively.
CONCLUSIONSNGF can improve the conductive function of injured peripheral nerve and facilitate regeneration of nerve.
