Study in vitro of populating autogenous Schwann cells into chemical extracted allogenous nerve.
- Author:
Jianyun WANG
1
;
Xiaolin LIU
;
Jiakai ZHU
;
Wenping LI
;
Jianping XIANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Transplantation; Cells, Cultured; Graft Survival; Immunohistochemistry; In Vitro Techniques; Male; Models, Animal; Nerve Regeneration; physiology; Peripheral Nerves; surgery; Rats; Rats, Inbred F344; Schwann Cells; transplantation; Sciatic Nerve; transplantation; Sensitivity and Specificity; Tissue Transplantation; Transplantation, Homologous
- From: Chinese Journal of Traumatology 2002;5(6):326-328
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo look for an ideal substance to repair large gap of nerve defect after injury by culture of Schwann cells (Scs) and preparation of acellular allogenous nerve grafts (ANG) with chemical extraction.
METHODSThe double adhesion culture and Arab-c to prohibit the fibroblast growth were used to achieve high-purified Scs. Triton-x-100 and sodium deoxycholate were used to achieve ANG. Finally the Scs were microinjected into the acellural nerve grafts and cultured in vitro. The consequence was analysed.
RESULTSHigh purified Scs and ANG were acquired, which could integrate each other well. Scs could survive and transfer to aline in vitro.
CONCLUSIONSPopulating Scs into chemical extracted ANG may be an ideal substance to repair the large gap of nerve defect after injury.
