Reciprocal action between BMP-2 and BMP-3 in cultured fibroblast in vitro.
- Author:
Guo-lin MENG
1
;
Yun-yu HU
;
Qin PU
;
Rong LU
;
Liu YANG
;
Jun WANG
;
Jian-ping BAI
;
Xin-zhi XU
;
Dan LI
Author Information
- Publication Type:Journal Article
- MeSH: Bone Morphogenetic Protein 2; Bone Morphogenetic Protein 3; Bone Morphogenetic Proteins; metabolism; Cells, Cultured; Fibroblasts; metabolism; Fracture Healing; physiology; Immunohistochemistry; Osteogenesis; physiology; Transforming Growth Factor beta
- From: Chinese Journal of Traumatology 2003;6(1):3-7
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo explore reciprocal action between BMP-2 (bone morphogenetic protein-2) and BMP-3 for better understanding of the mechanism of BMP during bone fracture union.
METHODSrhBMP-2 was added into the cultured fibroblasts with the concentration of 1,200 ng/ml. The expression of BMP-3 in fibroblasts was detected by immunohistochemistry. Eukaryotic expression vector pcDNA3-BMP-3 was transfected into the fibroblasts. After the effective expression of BMP-3 was identified, BMP-2 was also detected by immunohistochemistry in BMP-3 expression cells. The fibroblasts transfected with empty vector pcDNA3 were used as the control.
RESULTSExogenous rhBMP-2 could promote the expression of BMP-3 in fibroblasts. BMP-3 also could be detected in these cells.
CONCLUSIONSBMP-2 and BMP-3 could reciprocally adjust the expression in fibroblasts.
