Relationship between expression of Bax and Bcl-2 proteins and apoptosis in radiation compound wound healing of rats.
- Author:
Yü-fang CUI
1
;
Guo-wei XIA
;
Xiao-bing FU
;
Hong YANG
;
Rui-yun PENG
;
Ying ZHANG
;
Qing-yang GU
;
Ya-bing GAO
;
Xue-mei CUI
;
Wen-hua HU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; radiation effects; Female; Gamma Rays; Immunohistochemistry; Proto-Oncogene Proteins; biosynthesis; genetics; Proto-Oncogene Proteins c-bcl-2; biosynthesis; genetics; Rats; Rats, Wistar; Skin; pathology; radiation effects; Wound Healing; genetics; radiation effects; bcl-2-Associated X Protein
- From: Chinese Journal of Traumatology 2003;6(3):135-138
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo study the relationship between the expression of Bax, Bcl-2 proteins, and apoptosis in radiation compound wound healing of rats.
METHODSApoptosis, Bax and Bcl-2 proteins were estimated by in situ terminal labeling (TUNEL) and immunohistochemical methods.
RESULTS(1) Changes of the apoptosis in wound healing showed three typical characteristics: early occurrence, high frequency and delayed disappearance after radiation to rats when compared with those of simple wound group, which might be an important reason for radiation-induced delayed wound healing. (2) The expression of Bax protein increased evidently with the increment of apoptosis and showed a good corresponding relationship with the apoptotic frequency in the process of wound healing. While the expression of Bcl-2 protein decreased obviously as the apoptosis reached a maximum and showed increasing tendency up to normal level when the apoptosis decreased distinctively.
CONCLUSIONSBax and Bcl-2 proteins play an important role in the apoptotic regulation of radiation compound wound healing in rats.
