Changes of pulmonary intercellular adhesion molecule-1 and CD11b/CD18 in peripheral polymorphonuclear neutrophils and their significance at the early stage of burns.
- Author:
Rong-bing JIN
1
;
Pei-fang ZHU
;
Zheng-guo WANG
;
Da-wei LIU
;
Ji-hong ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bronchoalveolar Lavage Fluid; chemistry; Burns; blood; immunology; Cell Adhesion; Immunohistochemistry; In Situ Hybridization; Intercellular Adhesion Molecule-1; analysis; Lung; blood supply; Macrophage-1 Antigen; analysis; Neutrophils; immunology; pathology; Peroxidase; analysis; RNA, Messenger; analysis; Rats; Time Factors
- From: Chinese Journal of Traumatology 2003;6(3):156-159
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the role of intercellular adhesion molecule-1 (ICAM-1) in the accumulation of polymorphonuclear neutrophils (PMN) in the lungs at the early stage of burns.
METHODSMyeloperoxidase content in lung tissues and bronchoalveolar lavage fluid (BALF) were detected. ICAM-1 and its mRNA expression in lung tissues were determined by immunohistochemical method and in situ hybridization. CD11b/CD18 expression on the peripheral PMNs was measured by flow cytometry.
RESULTSThe levels of myeloperoxidase in lung tissues and BALF after burn injury were markedly higher than those of control. Expression of ICAM-1 and its mRNA in the lung tissues and CD11b/CD18 on peripheral PMNs surface was significantly increased at 2, 6, 12, 24 h after burns.
CONCLUSIONSPMNs accumulation in the lungs is related to increased ICAM-1 expression on pulmonary microvascular endothelial cells and CD11b/CD18 expression on PMN at the early stage of burn injury.
