Expression of SALL4 gene in patients with acute and chronic myeloid leukemia.
10.7534/j.issn.1009-2137.2013.02.011
- Author:
Qin CHEN
1
;
Jun QIAN
;
Jiang LIN
;
Jing YANG
;
Yun LI
;
Cui-Zhu WANG
;
Hai-Yan CHAI
;
Xing-Xing CHEN
;
Zhen QIAN
;
Ji-Chun MA
;
Ming ZHANG
Author Information
1. Department of Hematology, the Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu Province, China.
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Aged;
Aged, 80 and over;
Case-Control Studies;
Female;
Gene Expression;
Humans;
Leukemia, Myelogenous, Chronic, BCR-ABL Positive;
genetics;
Leukemia, Myeloid, Acute;
genetics;
Male;
Middle Aged;
Transcription Factors;
genetics;
Young Adult
- From:
Journal of Experimental Hematology
2013;21(2):315-319
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to investigate the expression level of the SALL4 gene and its clinical significance in patients with acute myeloid leukemia (AML) and chronic myeloid leukemia (CML). Real-time quantitative PCR (RQ-PCR) was performed to detect the expression level of SALL4 mRNA in bone marrow mononuclear cells (BMMNC) from 35 AML, 12 CML patients and 24 iron deficiency anemia patients as controls. The results indicated that the expression level of SALL4 in AML (0%-14%, median 1.43%) was obviously higher than that in controls (0% - 1%, median 0%) (P < 0.001). SALL4 expression was positive in 65.7% (23/35) AML patients. The frequency of SALL4 expression was in M2 (86.7%, 13/15) > M3 (75.0%, 6/8) > M1 (60.0%, 3/5) > M4 (14.3%, 1/7), and the difference among 4 groups was statistically significant (P = 0.008); there was no correlation of the frequency of SALL4 expression with the age, sex, white blood cell WBC count, hemoglobin concentration, platelet count and chromosomal abnormalities of AML patients (P > 0.05). All the 13 CML cases showed positive expression of SALL4 gene (1% - 128%, median 19.39%), which was higher than that in controls (P < 0.001). The analysis of receiver operating characteristic (ROC) curve showed the area under ROC curve (AUC) of AML and CML were 0.983 (95% confidence interval: 0.95 - 1.017) and 0.997 (95% confidence interval: 0.986 - 1.007) respectively. It is concluded that SALL4 expression is a common molecular event and can be considered as a molecular marker for assisting diagnosis of AML and CML.
