2-DG enhances TRAIL-induced apoptosis of leukemia HL-60 cells.
10.7534/j.issn.1009-2137.2013.02.019
- Author:
Su-Rong ZHAO
1
;
Hai-Feng DUAN
;
Pei ZHANG
;
Hao LIU
;
Chen-Chen JIANG
;
Zhi-Wen JIANG
Author Information
1. Anhui Engineering Technology Research Center of Biochemical Pharmaceuticals, Anhui Province, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Caspase 3;
metabolism;
Deoxyglucose;
pharmacology;
HL-60 Cells;
Heat-Shock Proteins;
metabolism;
Humans;
Nuclear Pore Complex Proteins;
metabolism;
RNA-Binding Proteins;
metabolism;
TNF-Related Apoptosis-Inducing Ligand;
metabolism;
pharmacology
- From:
Journal of Experimental Hematology
2013;21(2):351-355
- CountryChina
- Language:Chinese
-
Abstract:
This study was purposed to investigate the effects of 2-deoxy-D-glucose (2-DG) on sensitizing HL-60 cells to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis and its possible mechanism. The proliferative inhibition of HL-60 cells treated with different concentrations of 2-DG and TRAIL was measured by MTT assay. The cells were treated with 2-DG, TRAIL, and 2-DG combined with TRAIL at the concentration < IC50 value, i.e. 10 mmol/L for 2-DG and 100 ng/ml for TRAIL. Apoptosis was analyzed by flow cytometry with PI staining; the expression of RIP1, GRP78, and PARP was analyzed by Western blot; the activity of caspase-3 was detected by special detection kit. The results showed that the combined treatment of HL-60 cells for 48 h induced an apoptotic rate of (45.1 ± 4.3)%, which was significantly higher than that of treated with 2-DG or TRAIL alone; at the same time, the combined treatment potentiated the expression of GRP78 and caspase-3 activity, and down-regulated the expression of RIP1. It is concluded that 2-DG can sensitize HL-60 cells to TRAIL-induced apoptosis, which may be correlated with excessive endoplasmic reticulum stress response, down-regulation of RIP1, and increase of caspase-3 activity.
