Quantitative monitoring of multi-donor chimerism after multi-donor allogeneic hematopoietic stem cell transplantation.
10.7534/j.issn.1009-2137.2013.02.037
- Author:
Yu-Feng FENG
1
;
Xiang ZHANG
;
Guang-Hua CHEN
;
Yang XU
;
Fei-Ran GONG
;
Zi-Ling ZHU
;
Li-Jun DAI
;
Tie-Mei SONG
;
Jia-Zi ZHOU
;
Xiao-Wen TANG
;
Hui-Rong CHANG
;
Jing-Cheng MIAO
;
De-Pei WU
Author Information
1. The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, China.
- Publication Type:Journal Article
- MeSH:
Alleles;
Electrophoresis, Capillary;
Hematopoietic Stem Cell Transplantation;
Humans;
Polymerase Chain Reaction;
Postoperative Period;
Tissue Donors;
Transplantation Chimera;
genetics;
Transplantation, Homologous
- From:
Journal of Experimental Hematology
2013;21(2):436-440
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to establish a model for detecting the donor chimerism rate following the multi-donor hematopoietic stem cell transplantations, and simplify its calculation method. Patients with hematologic disease receiving allogeneic hematopoietic stem cell transplantation including single-donor and multi-donor were selected in this study and the donor cell chimerism rates were detected, using STR-PCR combined with capillary electrophoresis. The results indicated that the peaks of the sister alleles coming from the same individual were confirmed to have the approximate areas and can be replaced each other in the situation of mixed chimerism. In the calculation model, the value between reference chimerism and approximate chimerism have no significant difference using the hypothetical peak areas, and the result was confirmed to be accepted basing on typical measurement error between sister alleles (5% - 20%). It is concluded that the areas of share peaks can be replaced by non-share peaks and this conclusion can be used to calculate the double-donor CHM (DD-CHM)(%). Compared to the D alleles, R alleles show more strategic importance because it can lead to more accurate result and allowed simplifying the arithmetic calculations for DD-CHM(%).
