Expression of endothelial nitric oxide synthase mRNA in human RBCs during storage.
10.7534/j.issn.1009-2137.2013.02.047
- Author:
Xue CHEN
1
;
Chang-Hua ZHOU
;
Ying HONG
;
Tian-Xiang GONG
Author Information
1. Blood Research Laboratory, Chengdu Blood Center, Sichuan Province, China.
- Publication Type:Journal Article
- MeSH:
Blood Donors;
Blood Preservation;
Erythrocytes;
metabolism;
Humans;
Nitric Oxide Synthase Type III;
genetics;
metabolism;
RNA, Messenger;
genetics;
metabolism
- From:
Journal of Experimental Hematology
2013;21(2):481-483
- CountryChina
- Language:Chinese
-
Abstract:
This study was purposed to identify endothelial nitric oxide synthase (eNOS) mRNA in human RBCs during storage and to investigate the relationship of its changing profile and preservation time at 4°C. RT-PCR and gene sequencing were applied to identify eNOS-mRNA in banked RBC. Real time PCR was used to study the relationship of eNOS-mRNA expression and preservation time. The results showed that eNOS mRNA was detected in RBC. Compared with fresh RBC, the content of eNOS mRNA in RBC was 0.868 ± 0.119 stored for 1 week, which was 0.379 ± 0.289, 0.108 ± 0.134, 0.141 ± 0.141, 0.125 ± 0.12 stored for 2, 3, 4 and 5 weeks respectively. It is concluded that eNOS mRNA exists in human RBC and its content is decreasing gradually along with the prolongation of storage time in banked RBC. Stored for 3 weeks, the content of eNOS-mRNA remains to be at lower level of concentration in human RBC.
