Influence of TIEG1 on apoptosis of HL-60 cells and expression of Bcl-2/Bax.
10.7534/j.issn.1009-2137.2013.03.010
- Author:
Kun YAO
1
;
Ying YANG
;
Rong HU
;
Miao MIAO
;
Ai-Jun LIAO
;
Wei YANG
;
Zhuo-Gang LIU
Author Information
1. Department of Hematology, China Medical University, Shengyang, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Cell Proliferation;
drug effects;
Early Growth Response Transcription Factors;
pharmacology;
Gene Expression Regulation, Leukemic;
HL-60 Cells;
Humans;
Kruppel-Like Transcription Factors;
pharmacology;
Proto-Oncogene Proteins c-bcl-2;
metabolism;
bcl-2-Associated X Protein;
metabolism
- From:
Journal of Experimental Hematology
2013;21(3):587-590
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the influence of TIEG1 on apoptosis of HL-60 cells and the expression of Bcl-2/Bax. Different concentration of TIEG1 were used to treat HL-60 cells, the cell growth inhibition rate was detected by MTT method. After treating HL-60 cells with 12.03 ng/ml TIEG1, cell apoptosis was detected with flow cytometry. Bcl-2 and Bax was detected with RT-PCR. The results showed that TIEG1 had inhibitory effect on HL-60 cell proliferation, and in time-and dose-dependent manners. The more obvious inhibitory effect was observed in HL-60 cells treated with TIEG1 of 12.03 ng/ml. During the course of cell apoptosis, Bax expression increased, but Bcl-2 expression decreased (P < 0.05). It is concluded that TIEG1 inhibits HL-60 cell proliferation and induces apoptosis in time and dose-dependent manners. During the course of HL-60 cells apoptosis induced by TIEG1, Bcl-2/Bax are associated with HL-60 cell apoptosis induced by TIEG1.
