Effect of BCL11A gene on transcription of γ-globin gene.
10.7534/j.issn.1009-2137.2013.03.018
- Author:
Shun-Chang SUN
1
;
Zhi-Ming ZHOU
;
Chuan-Qing TU
;
Yun-Sheng PENG
;
Hui-Wen SONG
Author Information
1. Department of Clinical Examination, Bao'an District People Hospital, Shenzhen, Guangdong Province, China. bayysun@sina.cn
- Publication Type:Journal Article
- MeSH:
Carrier Proteins;
genetics;
Gene Expression Regulation, Leukemic;
Genes, Regulator;
Genetic Vectors;
Humans;
K562 Cells;
Nuclear Proteins;
genetics;
RNA Interference;
RNA, Small Interfering;
genetics;
Transcription, Genetic;
Transfection;
gamma-Globins;
genetics
- From:
Journal of Experimental Hematology
2013;21(3):628-632
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to explore the effect of BCL11A gene on transcription of γ-globin gene in K562 cells. B-cell lymphoma/leukemia 11A (BCL11A) gene was silenced by small interfering RNA (siRNA) expression vectors in K562 cells (human erythroblastic leukemia cell line). Gamma-globin mRNA level in K562 cells was determined by RT-PCR. Association between the BCL11A gene and γ-globin gene transcription was explored by comparison of mRNA levels. The results indicated that the silence rate of the BCL11A gene in K562 cells by 4 siRNA expression vectors was 49.7%, 55.4%, 78.2%, and 84.1%, respectively. The siRNA expression vector with 84.1% silence rate was transfected into K562 cells, transcription level of γ-globin mRNA in K562 cells transfected with siRNA expression vector increased 2.4 times as compared with control K562 cells. It is concluded that level of γ-globin mRNA increases when the BCL11A gene is silenced. It indicates that the BCL11A gene may be a negative regulator for γ-globin gene expression.
