Relation of B7-H3 molecule expression in multiple myeloma with poor prognosis and bone destruction.
10.7534/j.issn.1009-2137.2013.03.020
- Author:
Dan-Dan ZHAO
1
;
Liang LIN
;
Qing GE
;
Zhen-Yu LI
;
Xu-Peng HE
;
Kai-Lin XU
;
Jin LU
;
Xiao-Jun HUANG
Author Information
1. Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu Province, China.
- Publication Type:Journal Article
- MeSH:
Adult;
Aged;
Aged, 80 and over;
B7 Antigens;
genetics;
metabolism;
Bone and Bones;
pathology;
Calcium;
blood;
Cell Line, Tumor;
Female;
Humans;
Male;
Middle Aged;
Multiple Myeloma;
diagnosis;
metabolism;
pathology;
Prognosis
- From:
Journal of Experimental Hematology
2013;21(3):637-642
- CountryChina
- Language:Chinese
-
Abstract:
This study was purpose to investigate the B7-H3 expression in multiple myeloma cell lines and CD138 cells of patients with multiple myeloma, and explore its clinical significance. Three myeloma cell lines (RPMI8226, U266 and H929) were used. Forty-five patients with multiple myeloma were enrolled in the study. The expression of B7-H3 was detected by flow cytometry and RT-PCR. The relationship between B7-H3 and clinical prognostic factor was analyzed. The results showed that (1)In myeloma cell lines, high expression of B7-H3 was seen in RPMI8226 (92.30 ± 1.1)% and U266 (79.03 ± 1.2)% but not in H929 cell line (4.26 ± 0.2)%. (2) Exogenous IL-6 had no effect on upregulation of B7-H3 in myeloma cell lines. (3) In multiple myeloma patients, the proportions of B7-H3 positive cells in newly diagnosed, remission and relapsed patients were (48.58 ± 33.593)%, (22.16 ± 18.853)%, and (57.65 ± 28.296)%, respectively. The difference between the newly diagnosed and remission patients, and remission and relapsed patients was significant (P = 0.023, P = 0.004). (4)High B7-H3 expression was correlated with high numbers of bone destruction and high levels of serum calcium (P = 0.027, P = 0.046, respectively). It is concluded that the relation of B7-H3 molecule expression with prognosis of multiple myeloma may be negative, but with degree of bone destruction is positive, thus the high expression of B7-H3 may correlated with disease progression and bone destruction of patients with multiple myeloma.
