Expression and significance of vitamin D and its receptor mRNA in the peripheral blood of initial immune thrombocytopenic patients.
10.7534/j.issn.1009-2137.2013.03.029
- Author:
Wei MU
1
;
Wei WANG
;
Zhong-Guang CUI
;
Ai-Hua SUI
Author Information
1. Department of Hematology, The Affiliated Hospital of Qingdao University Medical College, Qingdao, Shandong Province, China.
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Aged;
Case-Control Studies;
Cells, Cultured;
Female;
Humans;
Male;
Middle Aged;
RNA, Messenger;
genetics;
Receptors, Calcitriol;
blood;
Thrombocytopenia;
blood;
Vitamin D;
blood;
Young Adult
- From:
Journal of Experimental Hematology
2013;21(3):684-687
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effect of vitamin D in pathogenesis and clinical treatment of patients with immune thrombocytopenic (ITP), ELISA was used to detect the level of 25-hydroxylate vitamin D3[25(OH)D3] and 1,25-dihydroxyvitamin D3[1,25(OH)2D3] in peripheral blood from 45 ITP patients and 30 normal controls. Vitamin D receptor (VDR) mRNA expression was detected by RT-PCR. The results showed that the levels of 25(OH)D3 (10.6 ± 7.7 ng/ml) and 1,25(OH)2D3 (69.9 ± 29.0 pg/ml) in peripheral blood of the initial ITP patients were lower than those in peripheral blood of normal controls (13.7 ± 9.1 ng/ml, 87.3 ± 19.9 pg/ml) (P < 0.05). The expression of VDR gene obviously increased in peripheral blood of initial ITP patients (1.588 ± 0.127), as compared with that in peripheral blood of normal controls (1.055 ± 0.734) (P < 0.05). It is concluded that vitamin D level and its receptor expression may play an important role in ITP, and vitamin D and its similarities may be a new agent to treat patients with ITP.
