Different changes of serum cytokines following HLA-identical and HLA haploidentical non-myeloablative allogeneic hematopoietic stem cell transplantation.
10.7534/j.issn.1009-2137.2013.03.037
- Author:
Yue-Ying ZHAO
1
;
Yan KANG
;
Qi-Yun SUN
;
Zheng DONG
;
Tie-Qiang LIU
;
Mei GUO
;
Guang-Xie LIU
;
Hui-Sheng AI
Author Information
1. Department of Hematology, Chinese Academy of Military Medical Sciences, Beijing, China.
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Child;
Cytokines;
blood;
Female;
HLA Antigens;
immunology;
Haplotypes;
Hematopoietic Stem Cell Transplantation;
methods;
Humans;
Interleukin-10;
blood;
Interleukin-17;
blood;
Interleukin-4;
blood;
Interleukin-6;
blood;
Male;
Middle Aged;
Transplantation, Homologous;
Tumor Necrosis Factor-alpha;
blood;
Young Adult
- From:
Journal of Experimental Hematology
2013;21(3):721-727
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the expression difference of serum cytokines in 20 patients receiving HLA-identical nonmyeloablative allogeneic hematopoietic stem cell transplantation (iNAHSCT) and HLA-haploidentical nonmyeloablative allogeneic hematopoietic stem cell transplantation (hiNAHSCT). IL-2, IL-4, IL-6, IL-10, TNF-α, γ-IFN and IL-17 were detected by flow cytometric bead array before and on week 1, 2, 4 after transplantation respectively. The results showed that the IL-2 level was found to be up-regulated at week 1 and 2 after transplantation in iNAHSCT group and in hiNAHSCT group respectively, but there was no difference between these two groups (P > 0.05). The γ-IFN levels was up-regulated at week 4 after transplantation in above-mentioned two groups, but no difference was found between these two groups. The IL-4 level increased at week 2 and 1 after transplantation in iNAHSCT and hiNAHCT groups respectively, but the IL-4 level in iNAHSCT group was higher than that in hiNAHSCT group. The IL-6 level rose at week 1 and 2 after transplant in above mentioned groups respectively, and reached to peak level at week 4 after transplantation, but IL-6 level in hiNAHSCT was higher than that in iNAHSCT group (P < 0.02). The IL-10 level was up-regulated at week 1 and 2 in iNAHSCT and hiNAHSCT groups respectively, but the IL-10 level in iNAHSC was higher than that in hiNAHSCT group. The TNF-α level was up-regulated at week 1 in hiNAHSCT group, but at week 2 in iNAHSCT group after transplantation. The TNF-α level in hiNHASCT group was higher than that in iNAHSCT group (P < 0.01). The IL-17 level was up-regulated at week 1 and week 4 after transplantation in hiNAHSCT and iNAHSCT groups respectively, the IL-17 level in hiNAHSCT group was high as compared with that in iNAHSCT group. It is concluded that the serum cytokine levels are obviously up-regulated in iNAHSCT and hiNHASCT groups, and reach to peak level at week 4 after transplantation. The IL-6, TNF-α and IL-17 level up-regulated significantly in hiNAHSCT group, but the IL-4 and IL-10 level up-regulated significantly in iNAHSCT.
