Effect of Anti-CD44 Monoclonal Antibody A3D8 on Expression of AP-1 in HL-60 Cells.
- Author:
Jie LI
1
;
Jie YANG
1
;
Jun YUAN
1
;
Yan LI
1
;
Rui-Cang WANG
1
;
Su-Yun WANG
1
;
Hong-Ling HAO
2
Author Information
- Publication Type:Journal Article
- From: Journal of Experimental Hematology 2016;24(5):1360-1364
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effect of anti-CD44 monoclonal antibody A3D8 on expression of transcription factor AP-1 in acute myeloid leukemia cells.
METHODSAfter acute leukemia cell line HL-60 was treated by different concentrations of A3D8, the proliferation and cell cycle were detected by MTT and FCM respectively. The expressions of c-JUN and c-FOS at mRNA and protein level were detected by RT-PCR and Western Blot respectively.
RESULTSThe proliferation of HL-60 was inhibited by A3D8. The A3D8 treatment increased the percentage of G/Gcells. The expressions of c-JUN at mRNA and protein level were both decreased in HL-60 cells treated with A3D8. The expressions of c-FOS at mRNA and protein level in rapamycin treatment groups showed no statistically significant difference as compared with that in control group.
CONCLUSIONSA3D8 can affect the activity of AP-1 through inhibiting the expressions of c-JUN at mRNA and protein level.
