Analysis of Lymphocyte Subsets in Peripheral Blood of Patients with Aplastic Anemia or Hypoplastic Myelodysplastic Syndrome.

Yuan-fei LV; Zheng-yu Yan; Nai-yiao Chen; Jian-hui WU; Bin Liu; Guo-li Wang; Jing Wang

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Analysis of Lymphocyte Subsets in Peripheral Blood of Patients with Aplastic Anemia or Hypoplastic Myelodysplastic Syndrome.

Author: Yuan-Fei LV ¹ ; Zheng-Yu YAN ² ; Nai-Yiao CHEN ² ; Jian-Hui WU ¹ ; Bin LIU ³ ; Guo-Li WANG ⁴ ; Jing WANG ⁵
Author Information

1. Public Health College of North China University of Sciences and Engineering,Tangshan 063015,Hebei Province,China.
2. Department of Hematology, Affiliated Hospital of North China Univesity of Science and Engineering,Tangshan 063000,Hebei Province,China.
3. Department of Hematology, Blood Desease Hospital, Chinese Academy of Medical Sciences, Tanjing 300020, China. E-mail:binliuwcums@163.com.
4. Public Health College of North China University of Sciences and Engineering,Tangshan 063015,Hebei Province,China
5. Science and Information College of Hebei University of Enginetring,Handan 056038, Hebei Province,China.
Publication Type:Journal Article
From: Journal of Experimental Hematology 2016;24(5):1505-1510
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo explore the ratio of lymphocyte subsets in peripheral blood of patients with aplastic anemia (AA) and patients with hypoplastic myelodysplastic syndrome (hypo-MDS) patients and to evaluate their significance.
METHODSThe clinical data of 181 cases of AA and 111 cases of hypo-MDS from January 2008 to December 2014 were collected from Blood Diseases Hospital of Chinese academy of medical sciences, and then the differences of lymphocyte subsets and its effect in 2 groups were analyzed.
RESULTSCD4/CD8ratio, proportion of CD3cells and its subsets CD3CD4/CD3CD8cells in hypo-MDS group were not significant different from AA group (P>0.05). the proportion of CD3CD16/CD56NK cells and CD3CD57T-LGL cells in hypo-MDS group was significantly higher than that in AA group (P<0.05, P<0.01), but CD19B lymphocyte percentage in hypo-MDS patients was lower than that in AA patients (P<0.05). After dividing group according to CD4/CD8ratio, the ratios of CD3CD16/CD56NK cells and CD3/CD57T-LGL cells were higher only in normal CD4/CD8ratio group of hypo-MDS patients than those in AA patients, while the ratio of B lymphocytes was significant different in inverted CD4/CD8ratio group between hypo-MDS and AA patients. The CD19B lymphocyte ratio in hypo-MDS patients was significantly lower than that in AA patients (P<0.05). As well, the levels of erythrocytes and platelets in peripheral blood between hypo-MDS and AA patients only in normal CD4/CD8ratio group were significantly different, while the significant difference of WBC count and reticulocyte ratio were observed in high CD4/CD8ratio and non-inverted CD4/CD8ratio groups, respectively; the significant difference of bone marrow blast ratio and muture monocyte ratio was found in high CD4/CD8ratio group.
CONCLUSIONThe changes of lymphocyte subsets can be used as an reference indicator for differential diagnosis of hypo-MDS and AA. The comparative analysis of patients with these 2 kinds of diseases after dividing into subgroups according to ratio of CD4/CD8cells is beneficial to differentiat diagnosis.