Identification of A Novel HLA-B*13:92 Allele and Analysis of Its 3D Model Structure.

Yong-Feng PEI; Hui-Ni HUANG; Heng-Cong LI; Guo-Guang WU

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Identification of A Novel HLA-B*13:92 Allele and Analysis of Its 3D Model Structure.

Author: Yong-Feng PEI ¹ ; Hui-Ni HUANG ¹ ; Heng-Cong LI ¹ ; Guo-Guang WU ²
Author Information

1. Nanning Institute of Transfusion Medicine, Nanning 530021, Guangxi Zhuang Autonomous Region, China.
2. Nanning Institute of Transfusion Medicine, Nanning 530021, Guangxi Zhuang Autonomous Region, China. E-mail: guangwu@szonline.net.
Publication Type:Journal Article
From: Journal of Experimental Hematology 2016;24(5):1558-1562
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo identify a novel human leukocyte antigen (HLA) allele HLA-B*13:92 and analyze 3D model of HLA molecule.
METHODSPolymerase chain reaction sequencing-based (PCR-SBT) was used in routine HLA typing, the B locus typing results of one sample was one base mismatch with B*13:01:01, B*58:01:01 at locus 189, The Group Specific Sequencing Products (GSSP) which target at B*13 and B*58 were used to confirm difference between the new allele and highest homologous allele, then the new allele was modeled by Swiss-model to its 3D structure.
RESULTSThe sequencing results showed that the new allele with highest homologous allele B*13:01:01 was the difference in the second exon at position 189 C>A (codon 39 GAC>GAA), 39 Asp (D) was changed to Glu (E). The amino acid substitution at residue 39 of the HLA polypeptide was located in α-helices of antigenic peptide-biding region.
CONCLUSIONThis allele is a new HLA-B allele found in Chinese Guangxi Zhang population and has been designated as HLA-B*13:92 by the World Health Organization (WHO) HLA Nomenclature Committee.