OBJECTIVETo investigate the distribution of Colton and Diego rare blood group antigens of blood donors in Chinese Xinjiang minorities.

METHODSA multiplex PCR was applied to screen for alleles antigens Diand Coin 1020 randomly selected healthy donors of Chinese Xinjiang minorities by using each 5 samples mixed detection method. The samples in the positive pools were further tested individually. Furthermore, the positive samples, including Di/Diand Co/ Cogenotypes were tested via 2 PCR-SSP assays for high frequency allele Diand Coto get the rare genotypes Di, Co.

RESULTSAmong 1020 samples 12 cases with Coallele, 45 cases with Diand 1 case with Diwere identified.

CONCLUSIONThe frequencies of Diand Coalleles are 2.30% and 0.59%, respectively. The information of rare blood donors obtained from the screening can provide a reference for matched blood transfusion, and further enrich the National Rare Blood Bank of China.