Effects of Different Culture Systems on the Hematopoietic Differentiation Ability of iPS Cells.

Di Fan; Wen-yin HE; Xiao-hua Niu; Zhan-hui OU; Yu-chang Chen; Xiao-fang Sun

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Effects of Different Culture Systems on the Hematopoietic Differentiation Ability of iPS Cells.

Author: Di FAN ¹ ; Wen-Yin HE ¹ ; Xiao-Hua NIU ¹ ; Zhan-Hui OU ¹ ; Yu-Chang CHEN ¹ ; Xiao-Fang SUN ²
Author Information

1. The Third Hospital Affiliated to Guangzhou Medical University,Key Laboratory for Major Obstetric Diseases of Guangdong Province,Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes,Guangzhou 510150, Guangdong Province, China.
2. The Third Hospital Affiliated to Guangzhou Medical University,Key Laboratory for Major Obstetric Diseases of Guangdong Province,Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes,Guangzhou 510150, Guangdong Province, China. E-mail:xiaofangsun@gzhmu.edu.cn.
Publication Type:Journal Article
From: Journal of Experimental Hematology 2016;24(6):1863-1868
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo investigate the in vitro effects of different culture systems on hematopoietic differentiation ability of induced pluripotent stem (iPS) cells.
METHODTwo culture systems including E8 and mTESR(freeder-free medium), and the classical ES culture medium were chosen for culture of iPS cells. The iPS cells maintaining in above mentioning culcure systems were co-cultured with OP9 cells(murine bone marrow stromal cells) in vitro to be induced to differentiate into hematopoietic stem/progenitor cells. Flow cytometry and real-time quantitative PCR were used to detect the expression of specific hematopoietic markers and the effects of different culture systems on the differentiation of iPS in vitro.
RESULTiPS cultured in the 3 selected medium could be differentiated into hematopoietic stem cells. Efficiency of hematopoietic differentiation was up to 28.4% in classical ES culture system, which was significantly higher than that in E8 and mTESR system.
CONCLUSIONUnder the co-culture with OP9, iPS can differentiate into hematopoietic stem/progenitor cells, which shows higher efficiency when iPS maintained in the ES medium.