Effect of miR-221-specific inhibitor on the proliferation and apoptosis of human colorectal carcinoma cells.
- Author:
Wei WANG
1
;
Kai SUN
;
Cheng-Tang WU
;
Shang-Tong LEI
;
Jun-Jie ZENG
;
Ying-Jun WU
;
Guo-Xin LI
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; Cell Line, Tumor; Cell Proliferation; drug effects; Colorectal Neoplasms; pathology; Humans; MicroRNAs; antagonists & inhibitors; metabolism
- From: Journal of Southern Medical University 2011;31(4):674-677
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate miRNA-221 expression in human colorectal carcinoma (CRC) cells and the effects of miR-221-specific inhibitor on the proliferation and apoptosis of CRC cells.
METHODSFour human CRC cell lines (HT-29, Lovo, SW-480, and CaCO2) were examined for miRNA-221 expression using real-time Q-PCR. The specific 2,-methoxy-modified RNA oligonucleotides of miR-221 (anti-miR-221) were synthesized and transfected into Caco2 cells via liposome, and the changes in the expression of miR-221 in the cells were detected by real-time Q-PCR. The proliferation and apoptosis of the transfected CRC cells were detected using MTT assay and flow cytometry.
RESULTSThe 4 human CRC cells showed significantly upregulated expression of miR-221 compare with HUVECs (P<0.01). The miR-221-specific inhibitor, anti-miR-221, significantly inhibited the expression of miR-221 in Caco2 cells and suppressed the cell proliferation, causing also obvious cell apoptosis (P<0.01).
CONCLUSIONThe miR-221-specific inhibitor shows potent inhibitory effect on the growth of CRC cells, suggesting its value as a potential anti-tumor candidate for treatment of CRC.
