Identification of ubiquitously expressed transcript as the potential interactor of hepatitis B virus polymerase.

Author: Meng-Zhang CHEN ¹ ; Yi ZOU ; Jun-Wu LI ; Yue-Qin LI ; Hong-Jian LI ; Tian-Hong ZHOU
Author Information

1. Department of Bioengineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
Publication Type:Journal Article
MeSH: Gene Products, pol; metabolism; Hepatitis B virus; enzymology; Humans; Neoplasm Proteins; metabolism; Protein Interaction Mapping; Two-Hybrid System Techniques; Virus Replication
From: Journal of Southern Medical University 2011;31(4):686-689
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo investigate the function of hepatitis B virus polymerase (HBV Pol) in the viral life cycle by screening the proteins interacting with HBV polymerase.
METHODSThe HBV Pol gene was constructed into the pGBKT7 vector. GAL4 yeast two-hybrid system was used to screen the human liver cDNA library to obtain proteins which interacted with HBV Pol. GST-pull down assay was applied to confirm the protein interactions.
RESULTSUbiquitously expressed transcript (UXT) was selected by the yeast two-hybrid system. GST-pull down assay confirmed the in vitro interaction between HBV Pol and UXT.
CONCLUSIONSUXT is a potential interactor of HBV Pol, and this protein interaction may provide clues of the function of HBV Pol in HBV life cycle.