Determination of the content of phloridzin using reversed-phase high-performance liquid chromatography.
- Author:
Jian-Rong HE
1
;
Xue-Ying LIU
;
Qing-Wei WANG
;
Zi-Chen YE
;
Min CHANG
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Chromatography, Reverse-Phase; methods; Phlorhizin; analysis
- From: Journal of Southern Medical University 2011;31(4):711-713
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a reversed-phase high-performance liquid chromatographic (RP-HPLC) method for determination of phloridzin content.
METHODSA RP-HPLC method was established for determination of phloridzin using an Inertsil ODS-3 (4.6×150 mm, 5 µm) column with the detection wavelength of 288 nm, flow rate of 1.0 ml/min, and column temperature of 25 degrees celsius;.
RESULTSThe result showed that the phloridzin had a good linear relationship when its concentration ranged between 0.5988 and 89.72 µg/ml. The regression equation was Y=46.370 X-0.6728 (r=0.9999, n=3). The average recovery of phloridzin was 99.40% with the relative standard deviations (RSD) of 0.67%.
CONCLUSIONThis method is simple, quick and accurate for determination of phloridzin content.
