Frozen embryo transfer combined with intrauterine administration of autologous peripheral blood mononuclear cells for repeated implantation failure: report of 3 cases.
- Author:
Lei-Ning CHEN
1
;
Song QUAN
;
Hong LI
;
Si-Mei CHEN
;
Wei-Qing ZHANG
;
Chen LUO
;
Ya-Li SONG
;
Fu-Qi XING
Author Information
- Publication Type:Case Reports
- MeSH: Adult; Blastocyst; Cryopreservation; Embryo Implantation; Embryo Transfer; methods; Female; Fertilization in Vitro; methods; Humans; Monocytes; Pregnancy; Pregnancy Rate; Treatment Failure
- From: Journal of Southern Medical University 2011;31(4):724-726
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the efficacy of frozen-thawed embryo transfer combined with intrauterine administration of autologous peripheral blood mononuclear cells (PBMCs) in the treatment of repeated implantation failure (RIF).
METHODSPBMCs obtained from 3 patients with RIF on the day of follicle rupture (natural cycle) or when the endometrial thickness reached 8 mm (hormone replacement cycle) were cultured in the presence of HCG for 48 h. The cultured PBMCs, along with freshly isolated PBMCs, were administered into the uterine cavity of the patients. Vitrified cleavage-stage embryos or blastocysts transfer was performed on day 3 or 5, respectively.
RESULTSVitrified embryo or blastocyst transfer resulted in pregnancy and healthy live births in all the 3 patients.
CONCLUSIONFrozen-thawed embryo transfer combined with intrauterine administration of autologous PBMCs may be an effective and safe approach to the treatment of RIF and may improve the outcomes of assisted reproduction.
