Cisplatin induces drug resistance in human esophageal squamous carcinoma cell line EC109 by decreasing CTR1 protein expression.
- Author:
Le YU
1
;
Ming-hui CHEN
;
Chun-ping GU
;
Yi-lei LI
;
Jing WEN
;
Jian-hua FU
;
Chi-hin CHO
;
Shu-wen LIU
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Squamous Cell; metabolism; Cation Transport Proteins; metabolism; Cell Line, Tumor; Cisplatin; pharmacology; Down-Regulation; Drug Resistance, Neoplasm; drug effects; Esophageal Neoplasms; metabolism; Humans
- From: Journal of Southern Medical University 2011;31(5):801-804
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the mechanism of the development of cisplatin resistance in a human esophageal squamous carcinoma cell line.
METHODSThe cytotoxicity of cisplatin in the cisplatin-resistant resistant cell line EC109/CDDP and its parental cell line EC109 was measured by MTT assay. Whole-cell cisplatin accumulation and Pt-DNA adduct formation were determined by inductively coupled plasma mass spectrometry (ICP-MS). Western blotting was used to investigate the protein expression of full length PARP, cleaved PARP, and copper transporter 1 (CTR1).
RESULTSEC109/CDDP cells was more resistant to cisplatin-induced cytotoxicity and apoptosis than EC109 cells. Compared with EC109 cells, EC109/CDDP cells exhibited less cisplatin accumulation and Pt-DNA adduct formation with also decreased CTR1 protein expression.
CONCLUSIONCisplatin induces drug resistant phenotype by decreasing the protein level of CTR1, which controls cell accumulation and cytotoxic effect of cisplatin.
