Development of an amplified luminescent proximity homogeneous immunoassay kit for detecting human hepatitis B virus e antibody.
- Author:
Qiang MA
1
;
An HE
;
Zhi-ning DONG
;
Tian-cai LIU
;
Li-ping ZOU
;
Guan-feng LIN
;
Ming LI
;
Ying-song WU
Author Information
- Publication Type:Journal Article
- MeSH: Equipment Design; Hepatitis B Antibodies; blood; isolation & purification; Humans; Immunoassay; instrumentation; Luminescent Measurements; instrumentation; Reagent Kits, Diagnostic
- From: Journal of Southern Medical University 2011;31(5):810-812
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop an amplified luminescent proximity homogeneous immunoassay (AlphaLISA) kit for the detection of human hepatitis B virus e antibody (HBeAb).
METHODSThe neutralizing and competitive inhibition method was used to develop the AlphaLISA kit for detection of serum HBeAb.
RESULTSThe working range of the kit was 0.003-16 NCU/ml with a sensitivity up to 0.003 NCU/ml. The intra- and inter-assay coefficient of variation was 5.3% and 6.8%, respectively. The kit showed no cross-reaction with HBcAb, and comparison of the detection results with those of a commercially available Elecsys HBeAb kit (Roche) for 136 samples showed a correlation coefficient of 0.961.
CONCLUSIONThe AlphaLISA kit for HBeAb detection meets the clinical requirements for detection HBeAb in human serum.
