Effect of ultrasound on osteoprotegerin and receptor activator nuclear factor kappaB ligand expression during root resorption in rats.
- Author:
Zhi-feng LIU
1
;
Juan XU
;
Ling-ling E
;
Dong-sheng WANG
;
Dan-dan BEI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Male; Osteoprotegerin; metabolism; RANK Ligand; metabolism; Rats; Rats, Wistar; Root Resorption; diagnostic imaging; metabolism; Ultrasonography, Doppler, Pulsed
- From: Journal of Southern Medical University 2011;31(5):813-816
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the effect of pulsed ultrasound on the expressions of osteoprotegerin (OPG) and receptor activator nuclear factor kappaB ligand (RANKL) during root resorption in a mouse model of orthodontic tooth movement.
METHODSThirty-two male Wistar rats (6-8 weeks old) were randomly assigned into 4 equal groups, including the blank control group, two ultrasound exposure groups with daily local LIPUS stimulation (100 and 150 MW/cm(2)) for 10 days during mechanical loading, and the control group with mechanical loading but not LIPUS exposure. Nickel-titanium closed-coil springs were used to generate 100 g mesial force for 10 days to move the maxillary right first molars. The expression of OPG and RANKL proteins at the compression sites was detected by immunohistochemistry.
RESULTSUltrasound stimulation significantly up-regulated the expression of OPG and down-regulated RANKL expression (P<0.05). The expressions of OPG and RANKL showed significant differences between the two ultrasound exposure groups (P<0.05).
CONCLUSIONUltrasound stimulation might be useful to protect against root resorption and accelerate its repair by regulating the expressions of OPG and RANKL.
