Expression of dopamine receptor D2 in pulmonary carcinoma cells and effects of dopamine on the cell apoptosis.
- Author:
Yang-hong PENG
1
;
Jing-ming ZHANG
;
Nai-kang ZHOU
;
Shan WANG
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma; metabolism; pathology; Animals; Apoptosis; drug effects; Cell Line, Tumor; Dopamine; pharmacology; Flow Cytometry; Humans; Lung Neoplasms; metabolism; pathology; Mice; Mice, Inbred BALB C; Mice, Nude; Receptors, Dopamine D2; metabolism
- From: Journal of Southern Medical University 2011;31(6):975-978
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect the expression of dopamine receptor D2 in different pulmonary carcinoma cells and investigate the effect of dopamine in inducing apoptosis of A549 cells.
METHODSWestern blotting and RT-PCR were employed to detect the expression of dopamine receptor D2 in different pulmonary carcinoma cells (95D, H460, GLC-82, A549 and H446 cells). The apoptosis of A549 cells after a 6-hour exposure to 0.02%, 0.04%, 0.08% and 0.1% dopamine was analyzed by flow cytometry. The apoptosis-inducing effect of dopamine in vivo was also tested by intratumoral injection of 1% dopamine in 2 BALB/c-nu mice bearing A549 tumor xenograft using flow cytometry.
RESULTSThe presence of dopamine receptor D2 expression was detected by Western blotting and RT-PCR in 95D, H460, GLC-82, A549 and H446 cells. Flow cytometry detected obvious apoptosis of A549 cells following dopamine exposure in vitro in positive correlation to dopamine concentration. In the tumor-bearing mice, dopamine also showed an obvious apoptosis-inducing effect on A549 cells.
CONCLUSIONDopamine receptor D2 exists extensively in different pulmonary carcinoma cells. Dopamine may promote the apoptosis of pulmonary carcinoma cells through dopamine receptor D2.