Change of IL-22R1 expression in human airway smooth muscle cells in response to different stimulating agents.
- Author:
Zhan ZHANG
1
;
Ya-ling LUO
;
Li-li ZHOU
;
Wen-yan LAI
;
Jian XU
;
Zhen-xing LI
;
Dun-qiang REN
;
Han YE
;
Hao-hai ZHONG
Author Information
- Publication Type:Journal Article
- MeSH: Asthma; blood; Cell Line; Humans; Interferon-gamma; pharmacology; Interleukin-4; pharmacology; Myocytes, Smooth Muscle; drug effects; metabolism; RNA, Messenger; genetics; Receptors, Interleukin; metabolism; Transforming Growth Factor beta; pharmacology
- From: Journal of Southern Medical University 2011;31(6):979-982
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effects of serum of asthmatic patients, dexamethasone, interleukin-4 (IL-4), interferon-gamma (IFN-γ) and transforming growth factor-β (TGF-β) on the expression of interleukin-22 receptor 1 (IL-22R1) mRNA and protein in HASMCs in vitro.
METHODSIL-22R1 mRNA and protein expressions in HASMCs treated with different stimulating agents were measured by real-time PCR and Western blotting, respectively.
RESULTSIL-22R1 mRNA and protein expressions in HASMCs were significantly increased after stimulation by serum from asthmatic patients, but decreased after co-stimulation with dexamethasone. IL-22R1 mRNA and protein expressions in the cells both increased after stimulation by IL-4, IFN-γ and TGF-β.
CONCLUSIONIL-22R1 in HASMCs might be involved in the pathogenesis of asthma, and the therapeutic effect of dexamethasone on asthma is mediated, at least partially, by IL-22R1. The effects of IFN-γ, IL-4, and TGF-β on asthma may also be attributed to their actions on HASMCs.
