The comparison between the vascular endothelial cells special cdc42-deficient heterozygous mice and the non-knockout mice on lung tissue pathological change and vasopermeability in acute lung injury.
- Author:
Guo-dong HU
1
;
Ying-hua CHEN
;
Wan-cheng TONG
;
Yuan-xiong CHENG
;
Lin ZHANG
;
Lei ZHANG
;
Shao-xi CAI
Author Information
- Publication Type:Journal Article
- MeSH: Acute Lung Injury; pathology; Animals; Capillary Permeability; Endothelial Cells; pathology; Integrases; genetics; Lung; blood supply; pathology; Mice; Mice, Knockout; cdc42 GTP-Binding Protein; genetics
- From: Journal of Southern Medical University 2011;31(6):995-998
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare the change of lung tissue and vasopermeability between the vascular endothelial cells special cdc42-deficient heterozygous mice and the non-knockout mice in acute lung injury.
METHODSThe mice with vascular endothelial cell-specific expression of cre recombinase were crossed with cdc42(flox/flox) mice. The cdc42(flox/+)Cre(+/-) F1 offspring mice were crossed back with cdc42(flox/flox) mice, resulting in the F2 generation mice with three genotypes, namely cdc42(flox/+)Cre(+/-), cdc42(flox/flox)Cre(-/-) and cdc42(flox/+)Cre(+/-). The heterozygous mice with cdc42(flox/+)Cre(+/-) genotype were selected as the model mice, with the other two genotype groups as the control. After intratracheal instillation of 2 mg/kg LPS to induce acute lung injury, the mice were sacrificed to examine the lung pathologies, lung wet/dry ratio and lung microvascular permeability.
RESULTSThe heterozygous mice with cdc42 gene knockout (cdc42(flox/+)Cre(+/-)) showed no significant differences from the two control groups in the lung pathological score, lung wet/dry ratio or the lung microvascular permeability coefficient.
CONCLUSIONThere were no significant difference on lung tissue and vasopermeability between the vascular endothelial cells special cdc42-deficient heterozygous mice and the non-knockout mice.
