Detection and genotype analysis of sapovirus associated with sporadic diarrhea in Shenzhen in 2009.
- Author:
Yan-ge WANG
1
;
Ya-qing HE
;
Wen-long XIANG
;
Yu-xue LIAO
;
Qing CHEN
;
Shou-yi YU
;
Gui-fang HU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; China; epidemiology; Diarrhea; epidemiology; virology; Female; Genetic Variation; Genotype; Humans; Infant; Infant, Newborn; Male; Middle Aged; Phylogeny; RNA, Viral; genetics; Sapovirus; classification; genetics; isolation & purification; Young Adult
- From: Journal of Southern Medical University 2011;31(6):1017-1019
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo conduct an epidemiological and genotype analysis of sapovirus (SaV) associated with sporadic diarrhea in Shenzhen in the year 2009.
METHODSA total of 852 fecal samples were collected from sporadic cases of diarrhea in Shenzhen in 2009 and detected by reverse-transcription polymerase chain reaction (RT-PCR) using the primers of SLV5317/5749. The PCR products were analyzed with 1.5% agarose gel electrophoresis and sequenced to construct the phylogenetic tree.
RESULTSSixteen samples were found positive for SaV, with a positivity rate of 1.88%. Sequence analysis identified 8 isolates as SaV GI genotype (including 3 SaV GI.1 and 5 SaV GI.2), 7 as SaV GIV genotype, and 1 as GII genotype.
CONCLUSIONSSaV infection is present in Shenzhen with GI as the predominant genotype. This is the first report of SaV GIV strains in China, which differs from the strains of Anhui-A141 and Beijing-CHN99/BJ360, suggesting the genotypic variety of SaV infection in China.
