Primry research of separation and culture of adult hepatocytes.
- Author:
Hong-ling LIU
1
;
Shao-jie XIN
;
Shao-li YOU
;
Yan HU
;
Yi-hui RONG
;
Lin ZHOU
;
Yong-ping YANG
;
Zhi-wei LI
;
Zhen-wen LIU
;
Pan-yong MAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Culture Techniques; Cells, Cultured; Hepatocytes; metabolism; Humans; Liver; cytology; Liver, Artificial; trends; Research
- From: Chinese Journal of Experimental and Clinical Virology 2008;22(5):376-378
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the separation and culture method of adult hepatocytes.
METHODSThe isolated adult hepatocytes were cultivated by RPMI 1640 medium at 37 degrees C in vitro. The characteristics of the growing hepatocytes were observed. Their synthesis of urea was detected. The transformation efficiency and density's change of lidocaine were analyzed.
RESULTSHepatocytes were successful separated from adult liver. And they were cultivated in common condition and hollow fiber reactor. The functional capacity of hepatocytes was for lidocaine metabolism and urea excretion.
CONCLUSIONThe adult hepatocytes have been successful separated from liver. And they can be cultivated in common condition and hollow fiber reactor. And it could provide a great quantity and high activity of hepatocytes for bioartificial liver.
