Primry research of separation and culture of adult hepatocytes
10.3760/cma.j.issn.1003-9279.2008.05.019
- VernacularTitle:成人肝细胞的分离及培养的初步研究
- Author:
Hong-Ling LIU
1
;
Shao-Jie XIN
;
Shao-Li YOU
;
Yan HU
;
Yi-Hui RONG
;
Lin ZHOU
;
Yong-Ping YANG
;
Zhi-Wei LI
;
Zhon-Wen LIU
;
Pan-Yong MAO
Author Information
1. 解放军第302医院
- Keywords:
Hepatocytes;
Cell culture;
Bioartificiul organs
- From:
Chinese Journal of Experimental and Clinical Virology
2008;22(5):376-378
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the separation and culture method of adult hepatocytes. Methods The isolated adult hepatocytes were cultivated by RPMI 1640 medium at 37℃ in vitro. The characteristics of the growing hepatocytes were observed. Their synthesis of urea was detected. The transformation efficiency and density's change of lidocaine were analyzed. Results Hepatocytes were successful separated from adult liver. And they were cultivated in common condition and hollow fiber reactor. The functional capacity of bepatocytes was for lidocaine metabolism and urea excretion. Conclusion The adult bepatocytes have been successful separated from liver. And they can be cultivated in common condition and hollow fiber reactor. And it could provide a great quantity and high activity of hepatocytes for bioartificiul liver.
