Clinical research of AFP variant with microcentrifugalcolumn method and crossed affinity immunoelectrophoresis autoradiography method.
- Author:
Yan CHEN
1
;
Ying-ying LIN
;
Min-hua HU
;
Yan-song CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Autoradiography; methods; Carcinoma, Hepatocellular; chemistry; Female; Humans; Immunoelectrophoresis; methods; Male; Middle Aged; Protein Isoforms; genetics; immunology; alpha-Fetoproteins; genetics; immunology
- From: Chinese Journal of Experimental and Clinical Virology 2008;22(5):379-381
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare the clinical value of microcentrifugalcolumn method with crossed affinity immunoelectrophoresis autoradiography method for the measurement of alpha-fetoprotein variant (AFP-L3) in differentiation of benign and malignant liver.
METHODSSerum AFP-L3 variants in 102 primary hepatocellular carcinoma patients and 41 chronic and cirrhosis patients were separated by microcentrifugalcolumn method and crossed affinity immunoelectrophoresis autoradiography method and the clinical value of both method were compared.
RESULTSIn 102 primary hepatocellular carcinoma patients, the sensitivity of AFP-L3 was 79.4% and 91.2% respectively, in 41 chronic and cirrhosis patients. The specificity of AFP-L3 was 70.7% and 29.3% respectively. The diagnostic accuracy was 76.9% and 73.4% respectively. The area in the ROC curve was 0.791 and 0.758 respectively. In the 4 primary hepatocellular carcinoma patients with lower AFP-L3, the AFP-L3 was positive by useing microcentrifugalcolumn method, but none of AFP-L3 were found by useing crossed affinity immunoelectrophoresis autoradiography method.
CONCLUSIONThe micro centrifugalcolumn method is more simple and rapid, it may be more useful in differentiation of benign and malignant liver than traditional crossed affinity immunoelectrophoresis autoradiography method.
