Clinical research of AFP variant with microcentrifugalcolumn method and crossed affinity immunoelectrophoresis autoradiography method
10.3760/cma.j.issn.1003-9279.2008.05.020
- VernacularTitle:微量离心柱法与亲和交叉免疫电泳法检测甲胎蛋白异质体的研究
- Author:
Yan CHEN
1
;
Ying-Ying LIN
;
Min-Hua HU
;
Yan-Song CHEN
Author Information
1. 福建肿瘤医院
- Keywords:
Alpha-fetoproteins;
Centrifugation;
Carcinoma;
bepaficellular;
Immunoelectrophoresis
- From:
Chinese Journal of Experimental and Clinical Virology
2008;22(5):379-381
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the clinical alue of microcentrifugalcolumn method with crossed affinity immunoelectrophoresis autoradiography method for the measurement of alpha-fetoprotein variant (AFP-L3) in differentiation of benign and malignant liver. Methods Serum AFP-L3 variants in 102 primary hepatocellular carcinoma patients and 41 chronic and cirrhosis patients were separated by microcentrifugalcolumn method and crossed affinity immunoelectrophoresis autoradiography method and the clinical value of beth method were compared. Results In 102 primary bepatocellular carcinoma patients, the sensitivity of AFP-L3 was 79.4% and 91.2% respectively, in 41 chronic and cirrhosis patients. The specificity of AFP-L3 was 70.7% and 29.3% respectively. The diagnostic accuracy was 76.9% and 73.4% respectively. The area in the ROC curve was 0.791 and 0.758 respectively. In the 4 primary hepatocellular carcinoma patients with lower AFP-L3, the AFP-L3 was positive by useing mierocentrifugalcohinm method, but none of AFP-L3 were found by useing crossed affinity immunoelectrophoresis autoradiography method. Conclusion The micro centrifugalcolunm method is more simple and rapid, it may be more useful in differentiation of benign and malignant liver than traditional crossed affinity immunoelectrophoresis autoradiography method.
