Evaluation of Direct Immunofluorescence Test with PCR for Detection of Novel Influenza A (H1N1) Virus during 2009 Pandemic.
10.3349/ymj.2011.52.4.680
- Author:
Jae Hoon LEE
1
;
Sae Ron SHIN
;
Ji Hyun CHO
Author Information
1. Department of Internal Medicine, Wonkwang University College of Medicine, Iksan, Korea. john7026@wku.ac.kr
- Publication Type:Brief Communication ; Evaluation Studies ; Research Support, Non-U.S. Gov't
- Keywords:
Influenza A (H1N1);
direct immunofluorescence assay
- MeSH:
Adolescent;
Adult;
Age Factors;
Body Temperature;
Child;
Fever/virology;
*Fluorescent Antibody Technique, Direct;
Humans;
Influenza A Virus, H1N1 Subtype/genetics/*isolation & purification;
Influenza, Human/epidemiology/*virology;
Pandemics;
*Polymerase Chain Reaction;
Sensitivity and Specificity
- From:Yonsei Medical Journal
2011;52(4):680-682
- CountryRepublic of Korea
- Language:English
-
Abstract:
During the 2009 novel influenza (H1N1) pandemic, the sensitivity of direct immunofluorescence assay (DFA) for H1N1 infection was 62% (266/429) of that of the polymerase chain reaction (PCR) test. The sensitivity of the DFA differed significantly with the age of patients: the sensitivity was the highest (71.8%) for patients aged <10 years and the lowest for patients aged > or =30 years. The sensitivity of DFA in patients aged > or =30 years was 40.7%. Furthermore, the sensitivity (67.3%, 171/254) of DFA was higher for patients who had a high temperature at admission. An increase in the incidence of H1N1 infection did not influence the sensitivity of DFA (62.1% vs. 62%; p=0.984) test, but resulted in a decrease in the negative predictive value, from 92.4% (700/757) to 69.6% (247/355). PCR may be useful as the initial test for diagnosing H1N1 infection in patients aged > or =30 years with a normal temperature at presentation.
