Expression of recombinant rubella virus E1 protein and initial application for detecting of antibody.
- Author:
Yao YI
1
;
Min-zhuo GUO
;
Tao YU
;
Wen-bo XU
;
Jin-ye YANG
;
Si-yong CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibodies, Viral; analysis; immunology; Cercopithecus aethiops; China; Cloning, Molecular; Escherichia coli; genetics; Gene Expression; Genetic Vectors; Humans; Recombinant Proteins; genetics; immunology; Rubella virus; genetics; immunology; Vero Cells; virology; Viral Envelope Proteins; genetics; metabolism
- From: Chinese Journal of Experimental and Clinical Virology 2008;22(5):382-384
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo apply recombinant rubella virus envelope protein-1 (E1) to detect human rubella virus IgG antibody.
METHODSRubella virus E1 protein was expressed in E. coli, purified E1 protein was used as the antigen for the detecting of anti rubella in human sera in the way of enzyme linked Immunosorbant assay (ELISA).
RESULTSThe antigenicity of the recombinant protein was checked by WHO rubella sera panel. We detected 200 sera samples, which came from Guangxi Guilin. 93% of these samples were positive.
CONCLUSIONThe antigenicity of recombinant E1 is a satisfied candidate antigen for the detecting of human rubella virus antibody. The prevalence of anti rubella virus IgG in Guangxi is 93%. It is at the some level compared with other provinces in China.
