Expression of recombinant rubella virus E1 protein and initial application for detecting of antibody

VernacularTitle:重组风疹病毒外膜蛋白E1的表达及其在血清学中的初步应用
Author: Yao YI ¹ ; Min-Zhuo GUO ; Tan YU ; Wen-Bo XU ; Jin-Ye YANG ; Si-Yong CHEN
Author Information

1. 中国疾病预防控制中心病毒病预防控制所
Keywords: Rubella virus; Gene expression; Enzyme-linked immunosorbent; Epidemiology
From: Chinese Journal of Experimental and Clinical Virology 2008;22(5):382-384
CountryChina
Language:Chinese
Abstract: Objective To apply recombinant rubella virus envelope protein-1 (E1) to detect human rubella vires IgG antibody .Methods Rubella virus E1 protein was expressed in E. coli., purified E1 protein was used as the antigen for the detecting of anti rubella in human sera in the way of enzyme linked Immunosorbant assay (ELISA) .Results The antigeneity of the recombinant protein was checked by WHO rubella sera panel. We detected 200 sera samples, which came from Guangxi Guilin. 93% of these samples were positive.Conclusion The antigenicity of recombinant E1 is a satisfied candidate antigen for the detecting of human rubella virus antibody. The prevalence of anti rubella virus IgG in Guangxi is 93 %. It is at the some level compared with other provinces in China.