Application of a simple method for the detection of measles virus genome by Loop-mediated isothermal amplification (LAMP)

VernacularTitle:应用简便逆转录环介导等温扩增方法检测麻疹病毒核酸
Author: Jian-Hui ZHOU ¹ ; Xiang HOU ; Chao CHEN ; Shuang WANG ; Xin CHANG ; Gui-Yan LIU ; Zhao-Nan WANG ; Song-Tao XU ; Yi-Xin JI ; Wen-Bo XU
Author Information

1. 吉林省疾病预防控制中心
Keywords: Loop-mediated isothermal amplificatcon; Measles virus; Nucleic acids
From: Chinese Journal of Experimental and Clinical Virology 2008;22(6):403-405
CountryChina
Language:Chinese
Abstract: Objective A new simple RT-LAMP method was applied to detect measles vires nucleic acid and compared with nest-RT-PCR. Methods Compare the detection rate of the RT-LAMP method with that of nest-RT-PCR by detecting measles virus nucleic acid from measles virus and clinical samples. Results The nucleic acid positive rotes of all 23 strains of measles virus are all 100% by the two methods. But to the detection of 18 clinical samples which are negative in measles isolation,the nest-RT-LAMP showed 56.52 % positive rate of nucleic acid of measles virus and nest-RT-PCR showed 47.83 %. Conclusion RT-LAMP is more sensitive than nest-RT-PCR.