Measles pathogenic surveillance from 2005 to 2007 in Guangdong Province
10.3760/cma.j.issn.1003-9279.2008.06.003
- VernacularTitle:广东省2005-2007年麻疹病原学监测
- Author:
Leng LIU
1
;
Huan-Ying ZHENG
;
Xue GUO
;
Jian-Qiong ZHU
;
Yi-Xin JI
;
Wen-Bo XU
Author Information
1. 广东省疾病预防控制中心
- Keywords:
Measles;
Virus isolation;
Success rate of virus isolation
- From:
Chinese Journal of Experimental and Clinical Virology
2008;22(6):406-408
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop pathogenic surveillance on measles and to effectively isolate measles virus. To know the genetic characterizations and molecular epidemiology of wildtype measles viruses from 2005 to 2007 in Guangdong Province,and provide the scientific basis for measles control and eradication. Methods Vero/ Slam cell line were used,measles viruses were isolated from throat swabs or urine specimens collected from uspected measles patients in outbreaks and sporadic patienta. A 450 nucleotides fragment of the C-terminal of the nucleoprotoin (N) gene was amplified and by RT-PCR and subjected to scquenee and phylngenetie analysis using Bio-Edit software. Results 82 wild-type measels virus were obtained from 377 throat swabs and urine specimens from 2005 to 2007 in Guangdong Province measles lab. The measles isolation rate was 23.58 % in 2005,17.11% in 2006,39.13% in 2007.The succeed rate of virus isolation is related to the quality of specimens collected and the days after rash occurrence. Conclusions We have grasped the techniealability of measles virus isolation and confirm action,and got higher isolation ratio. The wild-type measles virus isolated from Guangdong Province is of H1 genotype from 2005 to 2007,which is the same as the dominant genotype circulation.
