Immune response induced by recombinant adenovirus combined with recombinant adeno-associated virus type 1 containing HPV16 LI gene
10.3760/cma.j.issn.1003-9279.2008.06.006
- VernacularTitle:含HPV16L1基因重组腺病毒和1型重组AAV载体联合免疫效果的研究
- Author:
Yu-Bai ZHOU
1
;
Ze-Lin LI
;
Ling ZHOU
;
Wang SHENG
;
Hong-Tao MA
;
Yi ZENG
Author Information
1. 北京工业大学
- Keywords:
Papillomavirus,human;
Gene,L1;
Parvoviridae;
Adenoviridae
- From:
Chinese Journal of Experimental and Clinical Virology
2008;22(6):416-418
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the immune potency of recombinant adenovirus combined with rAAV1 vector expressing HPV16L1 protein in mice. Methods The rAdV and rAAV1 vector containing cedon-medified HPV16L1 gene was constructed using Admax and AAVmax packaging system respectively. C57 BL/6 mice were immunized with purified rAdV and rAAV1 vector through intramuscular and intranasal inoculation routes,and the titer of neutralizing antibody was determined by neutralization assay based HPV16 pseudovirus. Results Intramuscular immunization by rAAV1-med. HPV16L1 or combined with tAd-reed. HPV16L1 can induce higher titer of neutralizing antibody in serum than that of other groups. The titer of neutralizing antibody of intranasal groups is significantly lower than that of intramuscular group,although the prime-boost atrategy using in intranasal group was effective to enhance the specific humoral immunity. Conclusion The rAAV1-med. HPV16L1 combined with tAd-reed. HPV16L1 can induce higher titer of neutralizing antibody in serum through intramuscular route than that of other groups at the 16th week after the first immunization.
