Effect on mRNA and secretion levels of proinflammatory cytokines in DC infected by SARS-CoV N gene recombinant adenovirus
10.3760/cma.j.issn.1003-9279.2008.06.011
- VernacularTitle:SARS-CoV N基因重组腺病毒对树突状细胞前炎症细胞因子mRNA表达和分泌的影响
- Author:
Chun-Ling MA
1
;
Kun YAO
;
Feng ZHOU
Author Information
1. 山东医学高等专科学校
- Keywords:
Severe acute rispiratory syndreome,Coronavirus;
Nucleocapsid protein;
Dendritic cells;
Interleukin-6;
Tumor necrosis factor a;
Adenoviral vector
- From:
Chinese Journal of Experimental and Clinical Virology
2008;22(6):431-433
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the exact mechanism of SARS-CoV pathogenesis at the protein level. Method Under the condition of the establishment of dendrtic cells (DC) culture method,we used recombinant adenovirus to infect mature DC to make clear the development changes in mRNA levels and secreted protein levels of proinflammatory cytokines of IL-1β,IL-6,IL-8 and TNF-α by using RT-PCR and EL1SA. Result We found that mRNA levels and secreted protein levels of IL-6 and TNF-α in DC had increased gradually after rAd-N infection during fast 24 h compared with the control DC infected by rAd-LacZ( P < 0.05 or P < 0.01 ). Conclusion It is suggested that N protein may be related to the excessive secretion of prointlammatory cytokines during SARS-CoV infection at the acute phase.
