Molecular characterization and sequence comparison of the M and S segments of Hantavtrus ZJ5 strain

VernacularTitle:汉坦病毒ZJ5株M和S全片段的分子特性及序列的比较分析
Author: Ping-Ping YAO ¹ ; Han-Ping ZHU ; Fang XU ; Rong-Hui XIE ; Ling-Ling MEI ; Qun-Ying LU ; Zhi-Yong ZHU ; Xiao-Zhao DENG ; Yun ZHANG
Author Information

1. 浙江省疾病预防控制中心
Keywords: Hantavirus; Sequence analysis; Phylogenetic tree
From: Chinese Journal of Experimental and Clinical Virology 2008;22(6):434-436
CountryChina
Language:Chinese
Abstract: Objective In order to understand the molecular characters of Hantavirus ZJ5 strain,its complete M and S genome were sequenced and compared with that of other hantavirus strains. Methods We prepared the total RNA from ZJS. Infected cells and the raw or purified RT-PCR product was cloned and sequenced. Results With sequence compation,we found ZJ5 strain complete M and S segment had higher homology with SEO-type strains than other type of HV,but differential genes were 11.7%-19.2% and 6.7%-14.5% from SEOV.The phylogenefic trees constructed by complete M and S segment showed that ZJ5 strain was located in SEOV group,and structured alone embranchment. Conclusion The ZJ5 strain was believed to belong to SEO-type viras,and suggest that ZJ5 strain is a new subtype S SEOV greup,and structured alone embranchment. Conclusion The ZJ5 strain was beliceved to belong to SEO-type virus,and suggest that ZJ5 strain is a new subtype from other SEO viruses.