Improved expression of human rotavirus G1VP7 and G3VP7 antigens in the recombinant adenoviruses by codon optimization.
- Author:
Min WANG
1
;
Yi SHU
;
Jian-guo QU
;
Jian-wei WANG
;
Tao HONG
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; genetics; Antigens, Viral; genetics; metabolism; Cloning, Molecular; methods; Codon; genetics; Gene Expression; Genetic Vectors; genetics; Humans; Rotavirus; genetics; Transfection
- From: Chinese Journal of Experimental and Clinical Virology 2008;22(6):437-439
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo increase the recombinant adenovirus vector mediated human rotavirus G1VP7 and G3VP7 gene expression through coden optimization.
METHODSWe have artificially synthesized two genes of group A human rotavirus that encode G1VP7 and G3VP7 according to the human biased codon. The modified genes were transfected into 293 cells using adenovirus vectors and the gene products, the respective proteins were produced. The expression level of optimized genes and wild type genes was detected by Western Blot.
RESULTSA remarkable increase of the expression level of optimized G1VP7 and G3VP7 genes in comparison with the wild type control.
CONCLUSIONThe coden optimization indeed help increasing the recombinant adenovirus mediated human rotavirus gene expression, which indicated the potential applicationof such recombinant adenoviruses in the development of adenoviral-vectored rotavirus vaccines.
