Immune effects of mutated hepatitis B virus precore-core DNA vaccines in mice.
- Author:
Min ZHANG
1
;
Shao-jie XIN
;
Yan HU
;
Jun HOU
;
Hong-hui SHEN
;
Zhi-jie WANG
;
Pan-yong MAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Female; Hepatitis B; prevention & control; Hepatitis B Surface Antigens; genetics; Hepatitis B Vaccines; administration & dosage; Hepatitis B virus; genetics; Immunization; Male; Mice; Mice, Inbred BALB C; Mutation; Vaccines, DNA; administration & dosage; genetics; immunology
- From: Chinese Journal of Experimental and Clinical Virology 2008;22(6):446-448
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the immune effect of DNA vaccines encoding mutated HBV pre-c/c gene (VE2,VE4) in mice.
METHODSThree kinds of plasmid VEC(DNA vaccines encoding HBV pre-c/c gene), VE2 and VE4 were injected into the thigh muscles of different group of BALB/c mice.Blood and splenocytes from mice were isolated at 4 weeks after immunization. We also have mouse groups immunized with three of these plasmid combined with IFN-gamma gene plasmids. The anti-HBc and anti-HBe antibody in peripheral blood in mice were detected by enzyme linked immunosorbent assay (ELISA), antigen-specific cell immune responses were detected by CTL test and enzyme linked immunospot assay(ELISpot).
RESULTSWe found that anti-HBe titers of VE2 and VE4 immunizing groups are higher than VEC group (P < 0.05). We also observed that VE2 and VE4 could induce stronger antigen-specific immune responses than VEC and when combined with IFN-gamma plasmid,the antigen-specific immune responses are stronger than those without combination immunization in mice (P < 0.05).
CONCLUSIONSThe DNA vaccine VE2 and VE4 could induces stronger antigen-specific immune responses than VEC, and when combined with IFN-gamma plasmid,the antigen-specific immune responses are improved in mice.