Immune effects of mutated Hepatitis B Virus precore-core DNA vaccines in mice
10.3760/cma.j.issn.1003-9279.2008.06.016
- VernacularTitle:突变型乙肝病毒前C-C基因疫苗免疫BALB/C小鼠的研究
- Author:
Min ZHANG
1
;
Shao-Jie XIN
;
Yan HU
;
Jun HOU
;
Hong-Hui SHEN
;
Zhi-Jie WANG
;
Pan-Yong MAO
Author Information
1. 解放军第302医院
- Keywords:
Hepatitis B;
Vaccines,DNA;
Pre-C antigen;
Immanoengyme techniques;
Immunity,cellular
- From:
Chinese Journal of Experimental and Clinical Virology
2008;22(6):446-448
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the immune effect of DNA vaccines encoding mutated HBV pre-c/c gene(VE2,VFA) in mice. Methods Three kinds of plasmid VEC(DNA vaccines encoding HBV pre-c/c gene),VE2 and VE4 were injected into the thigh muscles of different group of BALB/c mice. Blood and splenocytes from mice were isolated at 4 weeks after immunization. We also have mouse groups immunized with three of these plasmid combined with IFN-γ gene plasmids. The anti-HBc and anti-HBe antibody in peripheral blood in mice were detected by enzyme linked immunusorbent assay( ELISA),antigen-specific cell immune responses were detected by CTL test and enzyme linked immunospot assay( ELISPOt). Results We found that anti-HBe titers of VE2 and VE4 immunizing groups are higher than VEC group( P < 0.05). We also observed that VE2 and VE4 could induce stronger antigen-specific immune responses than VEC and when combined with IFN-γ plasmid,the antigen-specific immune responses are stronger than those without combination immunization in mice (P < 0.05). Conclusions The DNA vaccine VE2 and VE4 could induces stronger antigen-specific immune responses than VEC,and when combined with IFN-γ plasmid,the antigen-specific immune responses are improved in mice.
