Vasoactive Intestinal peptide expression and its clinical significance In gastric adenocarcinoma
10.3760/cma.j.issn.1003-9279.2008.06.018
- VernacularTitle:VIP在胃腺癌中的表达及临床意义的研究
- Author:
Hui CHEN
1
;
Ping CEN
;
Jia-Quan LI
;
Yao-Guang LIN
;
Hai-Xin JIANG
;
Guo-Du TANG
;
Ning ZANG
;
Zhen-Bo FENG
;
Qi-Jian SU
;
Xin XIAO
Author Information
1. 广西医科大学附属第一医院
- Keywords:
Vasoactive intestinal peptide;
Stomach neoplasms;
Adenocarcinoma;
Pathology,Clinical
- From:
Chinese Journal of Experimental and Clinical Virology
2008;22(6):452-454
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of vasoactive intestinal peptide (VIP)in gastric adenocarcinoma,and to evaluate the correlation of VIP level with clinical pathologic parameters. Methods The level of VIP in sera from gastric adenocarcinoma patients and healthy people was investigated by ELISA. Moreover,the differential gene expression between gastric adenocarcinoma,gastric dysplasia,and the corresponding normal gastric mucosa were determined by RT-PCR. Western Blot was also used to measure the expression of VIP in the gastric odenouarcinoma and the normal gastric mucosa. Results The serum level of VIP was (5.794±0.014) ng/ ml in normal control and was (14.437±0.825) ng/ml in gastric adenocareinoma patients,showing significant difference (P<0.05). Meanwhile,the V/B of gastric adenocarcinoma tissues was greater than that of gastric dysplasia and the corresponding normal gastric mucosa (P < 0.01 ),the values of V/B were 1.5261±0.3028,0.9334±0.2872,and 0.9051±0.2794,respectively. The values of V/B between normal gastric mncesa and gastric dysplasia were not different significantly (P0.05).There were significantly negative correlation between the VIP mRNA expression of the differentiation degree of tumor( P < 0.05). The VIP mRNA expression was higher in gastric adenocarcinoma with lymph node metastasis than that without lymph node matsstsis (P<0.05).The VIP protein expression of the gastric adenouarcinoma tissues was greater than that of normal control. Conclusion This findings provide a direct evidence to support the possibility that VIP play a cofactor role in the pathogenesis of gastric adenccareinoma.
