Protection effects of acetylcholinesterase inhibitors on apoptosis.
- Author:
Jing HUANG
1
;
Yao YI
;
Sheng-li BI
;
Si-yong CHEN
;
Chuan-yue WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; drug effects; physiology; Cell Count; statistics & numerical data; Cell Proliferation; drug effects; Cell Survival; drug effects; Cercopithecus aethiops; Cholinesterase Inhibitors; pharmacology; Drug Antagonism; HeLa Cells; drug effects; pathology; Hepatitis B; pathology; Humans; Indans; pharmacology; Lipopolysaccharides; antagonists & inhibitors; toxicity; Piperidines; pharmacology; Tacrine; pharmacology; Vero Cells
- From: Chinese Journal of Experimental and Clinical Virology 2008;22(6):458-460
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate whether tacrine and donepezil can prevent apoptosis induced by Lipopolysaccharides.
METHODSPhase-contrast microscopes was used to observe the morphological changes of Vero cells. Cell counting kit-8 was used to measure cell survival vitro. DNA fragment was analyzed by agarose gel electrophoresis to determine the apoptotic biochemical changes.
RESULTSVero cells treated with 400 microg/ml and 500 microg/ml Lipopolysaccharides exhibited cell apoptosis. 10 micromol/L tacrine provided protective effect to 500 microg/ml Lipopolysaccharides induced cell apoptosis measured by Phase-contrast microscopes, cell counting kit-8 and DNA fragment analyze. However, donepezil did not show any protective effect of the apoptosis induced by 500 microg/ml Lipopolysaccharides.
CONCLUSIONLipopolysaccharides can induce apoptosis in Vero cells to built an apoptotic model in vitro. Tacrine rather than donepezil can inhibit Lipopolysaccharides induced apoptosis in Vero cells.
