Protection effects of Acetylcholinesterase inhibitors on apoptosis

VernacularTitle:乙酰胆碱酯酶抑制剂对乙型肝炎细胞凋亡的抑制作用
Author: Jing HUANG ¹ ; Yao YI ; Sheng-Li BI ; Si-Yong CHEN ; Chuan-Yue WANG
Author Information

1. 首都医科大学附属北京安定医院
Keywords: Hepatitis B; Apeptosis; Lipepolysaccharides; Tacrine; Donepezil
From: Chinese Journal of Experimental and Clinical Virology 2008;22(6):458-460
CountryChina
Language:Chinese
Abstract: Objective To investigate whether taerine and donepezil can prevent apeptosis induced by Lipopolysaecharides. Methods Phaze-contrast microscopes was used to observe the morphological changes of Vero cells.Cell counting kit-8 was used to measure cell survival vitro. DNA fragment was analyzed by agarose gel electrophoresis to determine the apeptotic biochemical changes. Results Veto cells treated with 400 μg/ml and 500 μg/ml Lipopolysaccharides exhibited cell apoptosis. 10 μmol/L tacrine provided protective effect to 500 μg/ml Lipepolysaecharides induced cell apoptosis measured by Phase-contrast microscopes,cell counting kit-8 and DNA fragment analyze. However,donepezil did not show any protective effect of the apeptosis induced by 500 μg/ml Lipopolysaecharides. Conclusion Lipopolysaecharides can induce apeptosis in Veto cells to built an apoptotic model in vitro. Tacrine rather than donepezil can inhibit Lipopolysaecharides induced apoptosis in Veto cells.