Detection of C-terminal 30 bp-deletion mutation of latent membrane protein 1 of EBV in childhood lymphoma.
- Author:
Zheng-de XIE
1
;
Chun-ju ZHOU
;
Lin WANG
;
Kun-ling SHEN
Author Information
- Publication Type:Journal Article
- MeSH: Base Sequence; Child; Gene Deletion; Herpesvirus 4, Human; classification; genetics; Humans; Lymphoma; genetics; Molecular Sequence Data; Phylogeny; Sequence Alignment; Viral Matrix Proteins; classification; genetics
- From: Chinese Journal of Experimental and Clinical Virology 2008;22(6):464-467
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study C-terminal 30 bp-deletion mutation of latent membrane protein 1 of the virus from childhood lymphoma.
METHODSNested-PCR was used to amplify C-terminal of EBV-LMP1 from childhood lymphoma and non-lymphoma associated to EBV, including Hodgkin lymphoma (HL), non-Hodgkin lymphoma (NHL)and reactive hyperplasia of lymph node (RL). Sequence analysis was performed on the positive PCR product.
RESULTSLMP1 with 30 bp deletion in C-terminal was detected in 11/25 HL,3/8 NHL and 5/15 RL cases respectively. There were no significant differences among HL, NHL and RL (P = 0.793). Sequence analysis showed that LMP1 detected in this study belongs to the following three subgroups: B95.8, China1 and China2.
CONCLUSIONLMP1 with 30 bp deletion in C-terminal widely existed in childhood HL,NHL and RL. There was no correlation between special types of LMP1 and the diseases. There were 3 LMP1 subgroups of EBV in children's lymphoma in the cases studied, including B95.8, China 1 and China 2.
