Detection of C-terminal 30 bp-deletion mutation of latent membrane protein 1 of EBV in childhood lymphoma

VernacularTitle:儿童淋巴瘤患者EBV-LMP1基因C末端30bp缺失突变检测
Author: Zheng-De XIE ¹ ; Chun-Ju ZHOU ; Lin WANG ; Kun-Ling SHEN
Author Information

1. 首都医科大学附属北京儿童医院
Keywords: Children,health services; Hodgkin disease; Genes,LMP1
From: Chinese Journal of Experimental and Clinical Virology 2008;22(6):464-467
CountryChina
Language:Chinese
Abstract: Objective To study C-terminal 30 bp-deletion mutation of latent membrane protein 1 of the virus from childhood lymphoma. Methods Nested-PCR was used to amplify C-terminal of EBV-LMP1 from childhood lymphoma and non-lymphoma associated to EBV,including Hodgkin lymphoma (HL),non-Hodgkin lymphoma (NHL)and reactive hyperplasia of lymph node (RL). Sequence analysis was performed on the positive PCR product.Results LMP1 with 30 bp deletion in C-terminal was detected in 11/25 HL,3/8 NHL and 5/15 RL cases respectively .There were no significant differences among HL,NHL and RL (P = 0.793 ). Sequence analysis showed that LMP1 detected in this study belongs to the following three subgroups: B95.8,China1 and China2. Conclusion LMP1 with 30 bp deletion in C-terminal widely existed in childhood HL,NHL and RL.There was no correlation between special types of LMP1 and the diseases. There were 3 LMP1 subgroups of EBV in children's lymphoma in the cases studied,including B95.8,Chinal and China 2.